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KMID : 0861120140180010083
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Korean Journal of Oriental Preventive Medicine
2014 Volume.18 No. 1 p.83 ~ p.92
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Antineoplastic Effect of Several Herbal Medicines on SNU-80 Anaplastic Thyroid Carcinoma Cell Line
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Yeo Hyun-Soo
Lee Min-Hye
Choi You-Kyung
Jeon Chan-Yong
Park Jong-Hyeong
Ko Seong-Gyu
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Abstract
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Objective : This study was performed to investigate the antineoplastic effect of Astragalus membranaceus,Angelica gigas, Rhus verniciflua Stokes and Trichosanthes kirilowii on SNU-80 anaplastic thyroid carcinomacell line.
Method : We examined whether our herbal medicines decreases cell growth rate of SNU-80 using MTTassay. We performed western blot analysis to verify that our herbal medicines induces apoptosis via caspasedependentmechanism. We also performed wound healing assay and transwell invasion assay to investigatewhether our herbal medicines affects the migration and invasion of anaplastic cancer cells, SNU-80. Wealso carried out ELISA assay to know our herbal medicines suppresses the expression of proinvasive molecules,such as VEGF and MMP-2 secreted from SNU-80.
Results : MTT assay demonstrates that Angelica gigas, Rhus verniciflua Stokes, and Trichosanthes kirilowiisuppressed strongly the growth of SNU-80. Western blot analysis demonstrates that Trichosanthes kirilowiiinduces apoptosis activating the cleavages of caspases (caspase-8, caspase-3) and PARP. Wound healingassay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibited the migration ofSNU-80. Transwell invasion assay demonstrates that Rhus verniciflua Stokes, and Trichosanthes kirilowii inhibitedthe invasion of SNU-80. Elisa assay demonstrates that Astragalus membranaceus, Angelica gigas, Rhusverniciflua Stokes, and Trichosanthes kirilowii suppressed the expression of VEGF and MMP-2.
Conclusion : We could conclude that several herbal medicines suppresses the growth and inhibits the migrationand invasion of SNU-80 which is anaplastic thyroid cancer cells. Especially, Rhus verniciflua Stokes,Trichosanthes kirilowii had stronger anti-cancer effect suggesting that we can apply them to treat anaplasticthyroid cancer.
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KEYWORD
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Astragalus membranaceus, Angelica gigas, Rhus verniciflua Stokes, Trichosanthes kirilowii, SNU-80, Antineoplastic Effect
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